An enzymatic colorimetric assay for glucose-6-phosphate

Enzymatic colorimetric assay of inorganic phosphate.

Vol.33, No.2, 1987 in June 1986, after recahibration of the glucose method, gave results equal to the survey mean: 640 and 1110 mg/L, respectively. The addition of two more calibration points solved our problem. However, we do not want to imply that five or more calibration points are required. The important question is not how many calibration points are used, but rather, are the concentration...

A colorimetric determination of inositol monophosphates as an assay for D-glucose 6-phosphate-1L-myoinositol 1-phosphate cyclase.

A rapid and convenient chemical assay for the enzyme d-glucose 6-phosphate-1l-myoinositol 1-phosphate cyclase is described. The 1l-myoinositol 1-phosphate formed enzymically was oxidized with periodic acid liberating inorganic phosphate, which was assayed. myoInositol 2-phosphate can be assayed in the same way. Glucose 6-phosphate and other primary phosphate esters gave only very small quantiti...

Homogeneous enzymatic colorimetric assay for total cysteine.

Patients with vascular disease have significantly higher concentrations of plasma total cysteine (tCYS) than do healthy individuals (1–7). The present method is a new, rapid, and sensitive enzymatic colorimetric assay for tCYS in plasma samples and is homogeneous in that it avoids separation methods. The tCYS assay uses only the recombinant enzymes methionine , -lyase (rMETase) and Sadenosylhom...

New enzymatic colorimetric assay for total homocysteine.

References 1. Linder MW, Prough RA, Valdes R. Pharmacogenetics: a laboratory tool for optimizing therapeutic efficacy. Clin Chem 1997;43:254–66. 2. Raimundo S, Fischer J, Eichelbaum M, Griese EU, Schwab M, Zanger UM. Elucidation of the genetic basis of the common ‘intermediate metabolizer’ phenotype for drug oxidation by CYP2D6. Pharmacogenetics 2000;10:577– 81. 3. Sachse C, Brockmoller J, Baue...

Glucose-6-phosphate Dehydrosgenase Deficiency: an Update